Product Details
»Book Publisher: John Wiley & Sons (17 March, 2004)
»ISBN: 0470848723
»Book author: Anne Ridley, Michelle Peckham, Peter Clark
»Amazon Rating:
Book Description:
Recent advances in molecular and biophysical techniques, particularly fluorescence and live cell imaging, are revolutionizing the study of cell motility. New bioprobes not only reveal simple intracellular localization, but also contain details of post-translational modifications, conformational state and protein-protein interactions. Coupling these insights with complementary advances in genetic and biochemical methods is enabling scientists to understand the processes involved in cell motility - from molecular motors to cell movements in vivo in a range of organisms and cell types.
This book features landmark essays that provide an up to date and fascinating account of current research and concepts in cell motility.These cover the roles of molecular motors that drive movement and their interactions with the cytoskeleton as well as membrane dynamics that allow cells to change shape and to move.
Cell motility plays a key role in development - there are chapters on the genetics of cell migration, the regulation of contact repulsion in growth cones, and the progression from cell migration to cell-cell adhesion. Cell motility is directional - experts describe the molecules that regulate chemotaxis, allowing cells to migrate along pathways specified by chemical gradients. Finally, cell motility can be perturbed by mutation–metastasis occurs when cells lose their normal intercellular interactions and invade other tissue types. All these processes are regulated by signals from the environment, including other tissues in the body, and the various molecules that transmit and transduce these signals are discussed.
This book is a ‘must read’ for cell biologists working in a variety of fields, from development to wound healing, at all levels - post-doctoral fellows, post-graduate students and lab technicians. It is also stimulating reading for molecular and developmental biologists, biophysicists and biochemists.
Hardcover: 258 pages
Publisher: Humana Press; 1st edition (January 15, 2001)
Language: English
ISBN-10: 0896037665
ISBN-13: 978-0896037663
Product Dimensions: 9.3 x 6.2 x 0.9 inches
"This is a concise but comprehensive book that would be a valuable reference for any researcher who wishes to study this important cytoplasmic motor protein. It would be a valuable book to have in the laboratory as well as a useful reference book in a life sciences library. "-Doody's Health Sciences Book Review Journal
Product Description
Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches considered are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed to successfully study the multifaceted world of kinesin-like proteins and to explore their many functions.
Product Details
»Book Publisher: Humana Press (March, 2000)
»ISBN: 0896036324
»Book author: John S. Elce
Book Description:
Review
"This book collects contributions from essentially all leading specialists in the area and the chapters are constructed in a way that should make it a useful bench-top companion. The book is good value for money: 36 chapters for $89 should make it an all but mandatory acquisition for libraries, and especially laboratories having programs on cell biology and cell pathology." -Cell Biology International
Product Description
John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of ยต- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions of, and the effects of inhibitors on, calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. Highly practical and readily reproducible, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and functions of calpains.
- Publisher: Humana Press; 2nd edition (September 5, 2008)
- Language: English
- ISBN-10: 1588294315
- ISBN-13: 978-1588294319
Editorial Reviews
Reviews of the first edition:
"This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates." - Doody's Health Sciences Book Review Journal
"Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs." -Teratology
"...this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. ...we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there." - TINS
"Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols....The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols." - The Quarterly Review of Biology
"This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists." - Journal of Endocrine Genetics
Product Description
This new edition offers a comprehensive guide to cutting-edge methods used today across the field of molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The book emphasizes the most important methods of experimental molecular biology and the particular advantages of each different species.